Multiplexed polymerase chain reaction in micropipette format
AssigneeRevvity Health Sciences, Inc.
InventorAndrew Brodsky
Life of this patent
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Abstract
A system may include a horizontal actuator to move a tray, to which a microwell plate and a microfluidic chip may be coupled. The system may include a vertical actuator to move a support arm, to which a plurality of pipettes or pipette tips may be coupled. The system may include a rotational actuator to move an angle bracket, to which a magnet may be coupled. The system may include a heater, through which the pipettes may extend. The system may include a pump to control the flow of fluids through the pipettes. Disclosed methods include performing PCR within the described system.
Claims12
A system, comprising:
- a first pipette including a first pipette tip at a first end of the first pipette and a second end of the first pipette opposite to the first pipette tip along a first length of the first pipette;
- a second pipette including a second pipette tip at a first end of the second pipette and a second end of the second pipette opposite to the second pipette tip along a second length of the second pipette;
- a three-way connector that fluidically couples the second end of the first pipette to the second end of the second pipette and fluidically couples the second ends of the first and second pipettes to a feeder conduit;
- and
- a heating element thermally coupled to at least a portion of the first and second lengths.
A system, comprising:
- a first pipette including a first pipette tip at a first end of the first pipette and a second end of the first pipette opposite to the first pipette tip along a first length of the first pipette;
- a second pipette including a second pipette tip at a first end of the second pipette and a second end of the second pipette opposite to the second pipette tip along a second length of the second pipette;
- a first three-way connector that fluidically couples the second end of the first pipette to the second end of the second pipette and fluidically couples the second ends of the first and second pipettes to a first feeder conduit;
- a third pipette including a third pipette tip at a first end of the third pipette and a second end of the third pipette opposite to the third pipette tip along a third length of the third pipette;
- a fourth pipette including a fourth pipette tip at a first end of the fourth pipette and a second end of the fourth pipette opposite to the fourth pipette tip along a fourth length of the fourth pipette;
- a second three-way connector that fluidically couples the second end of the third pipette to the second end of the fourth pipette and fluidically couples the second ends of the third and fourth pipettes to a second feeder conduit;
- a first heating element thermally coupled to at least a portion of the first and third lengths;
- and
- a second heating element thermally coupled to at least a portion of the second and fourth lengths.
Dependencies are inferred from the claim text.