Electroporation/electrochemotherapy of surgically sensitive regions

A system for vacuum-assisted superficial electroporation, comprising:

• a vacuum-assisted superficial electroporation probe, comprising:
• an electrode mounting support comprising an enclosed container, the electrode mounting support comprising a top side and a bottom side, the top side comprising a singular hole and a plurality of pairs of holes, the bottom side comprising a plurality of apertures, each respective aperture being aligned to one of the singular hole and a pair of holes of the plurality of pairs of holes, each respective pair of holes of the top side and the respective aperture of the bottom side receiving one electrode passing there through, a path extending along the singular hole and an aperture of the plurality of apertures located along with the singular hole forming a hollow path configured to a vacuum pressure being applied there through;
• and
• at least two electrodes, each respective electrode comprising a wire blade protruding out from the bottom side of the electrode mounting support through an aperture of the plurality of apertures, each respective wire blade comprising a U-shaped wire comprising a flat base with two parallel lateral edges extending upwards from the flat base, a first lateral edge of the two parallel lateral edges passing through a first hole of a pair of holes of the plurality of pairs of holes and a second lateral edge of the two parallel lateral edges passing through a second hole of the pair of holes of the plurality of pairs of holes, an electrically conductive wire being welded to a first free end of the U-shaped wire and a second free end of the U-shaped wire being fixed onto the top side of the electrode mounting support, the at least two electrodes configured to transfer a pulsed electric field to a plurality of target cells by putting the flat base of the wire blade in contact with a zone of a tissue comprising the plurality of target cells;
• an electrical pulse generator electrically connected to the at least two electrodes, an end of the electrically conductive wire being connected to the electrical pulse generator, the electrical pulse generator configured to apply a pulsed electric field to the at least two electrodes;
• a vacuum device, comprising:
• a vacuum tube comprising a flexible tube, the vacuum tube comprising a proximal end and a distal end, the distal end being fixed at a location on top of the hollow path, the vacuum tube configured to transfer a suction pressure to the plurality of target cells while the flat base of the wire blade being in contact with the zone of the tissue;
• and
• a vacuum pressure generator connected to the vacuum-assisted superficial electroporation probe via the vacuum tube, the proximal end of the vacuum tube being connected to the vacuum pressure generator, the vacuum pressure generator configured to apply the suction pressure through the vacuum tube;
• and
• a processing unit electrically connected to the electrical pulse generator and the vacuum pressure generator, the processing unit comprising:
• a memory having processor-readable instructions stored therein;
• and
• a processor configured to access the memory and execute the processor-readable instructions, which, when executed by the processor configures the processor to perform a method, the method comprising:
• tightening a contact between the respective flat bases of the at least two electrodes and the zone of the tissue by pulling the zone of the tissue towards the at least two electrodes via applying a vacuum pressure onto the zone of the tissue utilizing the vacuum pressure generator;
• and
• inducing electroporation to the plurality of target cells by applying at least one sequence of electric voltage pulses between the at least two electrodes utilizing the electrical pulse generator.