Methods of loading extracellular vesicles
AssigneeLonza Sales AG
InventorsKimberly Ellis, Aaron Noyes, Andrew Wood
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Abstract
The present disclosure relates to methods of loading an EV with a payload. In some aspects, the payload and the EV are mixed at a specific loading condition (e.g., at the disclosed salt concentrations, loading temperature, loading duration, payload feed concentration, and/or EV feed concentration), such that the amount of the payload that is associated with the exterior surface of the EV is increased. Also provided herein are methods for producing the extracellular vesicles and methods for using the extracellular vesicles to treat diseases or disorders.
Claims20
A method of loading a payload to an extracellular vesicle (EV) comprising mixing the payload with the extracellular vesicle in a loading buffer comprising a salt, wherein the salt comprises NaCl (sodium chloride) at a concentration of at least 140 mM to about 200 mM.
Dependencies are inferred from the claim text.