Ambient temperature lipid particle storage systems and methods

A process for vitrification of one or more particles above cryogenic temperature, the process comprising:

• a) placing a vitrification mixture comprising a particle thereof and a vitrification medium in or on a substrate comprising or forming a capillary network, and placing said substrate in a
• desiccation chamber, wherein the particle comprises:
• a viral capsid, viral envelope, or portion thereof;
• or a lipid particle;
• b) lowering an atmospheric pressure within the desiccation chamber;
• c) providing a heat energy to the particle, wherein the heat energy is sufficient to prevent the vitrification mixture from experiencing freezing conditions;
• d) desiccating the vitrification mixture by capillary action until the vitrification mixture enters a glassy state;
• e) storing the vitrification mixture for a period of at least three weeks at a temperature equal to or less than 60° C.;
• and
• f) reconstituting the particle in an aqueous medium, wherein the particle retains equivalent activity as the particle or contents thereof prior to step a).