Hydrogel systems for skeletal interfacial tissue regeneration applied to epiphyseal growth plate repair
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsJuan M. Taboas, Jingming Chen
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Abstract
Described herein are biomaterials, systems, and methods for guiding regeneration of an epiphyseal growth plate or similar interfacial tissue structures. In one aspect, the disclosed technology can include a biologic material that can comprise one or more of a hydrogel carrier for growth factors and MSCs, chondrogenic and immunomodulatory cytokines, microparticles for prolonged and spatially controlled growth factor delivery; and/or porous scaffold providing mechanical support. The implanted material can be applied via various different modalities depending on the nature of the physeal injury. One modality is an injectable hydrogel and another modality is an implantable hydrogel infused scaffold.
Claims20
A method of forming a layered biomaterial implant in an epiphyseal growth plate, comprising:
- injecting a hypertrophic hydrogel layer, a prehypertrophic hydrogel layer, and a proliferative hydrogel layer into a defect in the epiphyseal growth plate one layer at a time;
- and
- allowing or causing each of the hydrogel layers to solidify, thereby forming the layered biomaterial implant in the epiphyseal growth plate, wherein:
- each of the hydrogel layers comprise mesenchymal stem cells (MSCs);
- the hypertrophic hydrogel layer further comprises a thyroid hormone;
- the prehypertrophic hydrogel layer further comprises a transforming growth factor-beta protein (TGF-β);
- the proliferative hydrogel layer further comprises i) a TGF-β, and ii) an Indian hedge hog (IHH) receptor agonist or a parathyroid hormone related peptide (PTHrP) receptor agonist;
- and
- the prehypertrophic and proliferative hydrogel layers each comprise i) a bioinert hydrophilic polymer, ii) an amino acid polymer that comprises collagen sequences and cell-binding motifs and is biodegradable via enzymatic degradation, and iii) a polysaccharide that is biodegradable via enzymatic degradation.
Dependencies are inferred from the claim text.