Pumpless encapsulation of messenger RNA
AssigneeTranslate Bio, Inc.
InventorsShrirang Karve, Priyal Patel, Natalia Vargas Montoya, Frank DeRosa, Michael Heartlein
Life of this patent
Estimating the term…
Abstract
The present invention provides, among other things, a process of encapsulating messenger RNA (mRNA) in liposomes comprising a. providing a first stream comprising an mRNA solution at a first controlled flow rate, b. providing a second stream comprising a lipid solution at a second controlled flow rate, and c. mixing the first stream and the second stream to form mRNA-encapsulated liposomes, wherein the first controlled flow rate and the second controlled flow rate are achieved without use of a pump.
Claims11
A process of encapsulating messenger RNA (mRNA) in liposomes comprising:
- a. providing a first stream comprising an mRNA solution at a first controlled flow rate from a first reservoir through a first conduit,
- b. providing a second stream comprising a lipid solution at a second controlled flow rate from a second reservoir through a second conduit, and
- c. mixing the first stream and the second stream at a junction comprising a T connector or a Y connector to form mRNA-encapsulated liposomes,
- wherein the first controlled flow rate, the second controlled flow rate, and the mixing are achieved using gravity and atmospheric pressure without use of a pump, and wherein the process results in an encapsulation efficiency of at least 75%.
Dependencies are inferred from the claim text.