Bremia lactucae resistance SG01
AssigneeSyngenta Crop Protection AG
InventorsMichel De Lange, Anoma Akuvi Lokossou
Life of this patent
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Abstract
The present invention relates to novel lettuce plants displaying resistance to Bremia lactucae. The present invention also relates to seeds and parts of said plants, for example leaves and heads. The present invention further relates to methods of making and using such seeds and plants. The present invention also relates to novel genetic sequences associated with said resistance to Bremia lactucae and to molecular markers associated with said novel genetic sequences.
Claims11
A method for producing a lettuce plant with enhanced resistance to Bremia lactucae, the method comprising:
- a) crossing a Lactuca sativa plant with a lettuce plant to produce progeny plants, wherein:
- i) the Lactuca sativa plant has enhanced resistance to Bremia lactucae and comprises an introgressed sequence from Lactuca serriola that confers a qualitative and dominant resistance to Bremia lactucae, wherein said introgressed sequence is comprised in Lactuca sativa line 18LEN002364, representative seed of L. sativa 18LEN002364 having been deposited with the NCIMB under Accession No. NCIMB 43625, wherein the resistance of the plant to Bremia lactucae is enhanced as compared with a L. sativa plant lacking said introgressed sequence, and wherein said introgressed sequence is located on chromosome 2 and comprises one or more of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker SL1831 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker SL1847 in SEQ ID NO:
- 6;
- c) a G genotype in the heterozygous or homozygous state for SNP marker SL1887 in SEQ ID NO:
- 11;
- and/or
- d) a C genotype in the heterozygous or homozygous state for SNP marker SL1883 in SEQ ID NO:
- 16;
- and
- ii) the lettuce plant lacks said introgressed sequence conferring resistance to Bremia lactucae;
- and
- b) selecting a progeny plant comprising said introgressed sequence conferring resistance to Bremia lactucae, said selecting step comprising detecting one or more of the following SNP markers:
- i) a G genotype in the heterozygous or homozygous state for SNP marker SL1831 in SEQ ID NO:
- 1;
- ii) an A genotype in the heterozygous or homozygous state for SNP marker SL1847 in SEQ ID NO:
- 6;
- iii) a G genotype in the heterozygous or homozygous state for SNP marker SL1887 in SEQ ID NO:
- 11;
- and/or
- iv) a C genotype in the heterozygous or homozygous state for SNP marker SL1883 in SEQ ID NO:
- 16;
- thereby producing a plant with enhanced resistance to Bremia lactucae.
A method for producing a F1 hybrid lettuce plant with enhanced resistance to Bremia lactucae, the method comprising crossing a first inbred Lactuca sativa plant with a different inbred L. sativa plant to produce F1 hybrid progeny, wherein:
- i) the first inbred L. sativa plant has enhanced resistance to Bremia lactucae and comprises an introgressed sequence from Lactuca serriola that confers a qualitative and dominant resistance to Bremia lactucae, wherein said introgressed sequence is comprised in Lactuca sativa line 18LEN002364, representative seed of L. sativa 18LEN002364 having been deposited with the NCIMB under Accession No. NCIMB 43625, wherein the resistance of the plant to Bremia lactucae is enhanced as compared with a L. sativa plant lacking said introgressed sequence, and wherein said introgressed sequence is located on chromosome 2 and comprises one or more of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker SL1831 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker SL1847 in SEQ ID NO:
- 6;
- c) a G genotype in the heterozygous or homozygous state for SNP marker SL1887 in SEQ ID NO:
- 11;
- and/or
- d) a C genotype in the heterozygous or homozygous state for SNP marker SL1883 in SEQ ID NO:
- 16.
A method for identifying a lettuce plant with enhanced resistance to Bremia lactucae, said method comprising the step of detecting one or more of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker SL1831 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker SL1847 in SEQ ID NO:
- 6;
- c) a G genotype in the heterozygous or homozygous state for SNP marker SL1887 in SEQ ID NO:
- 11;
- and/or
- d) a C genotype in the heterozygous or homozygous state for SNP marker SL1883 in SEQ ID NO:
- 16;
- thereby identifying a lettuce plant with enhanced resistance to Bremia lactucae.
A method of producing lettuce seed, the method comprising growing a lettuce plant from a seed, and allowing the plant to produce further lettuce seed, wherein:
- i) the seed produces a Lactuca sativa plant that has enhanced resistance to Bremia lactucae and comprises an introgressed sequence from Lactuca serriola that confers a qualitative and dominant resistance to Bremia lactucae, wherein said introgressed sequence is comprised in Lactuca sativa line 18LEN002364, representative seed of L. sativa 18LEN002364 having been deposited with the NCIMB under Accession No. NCIMB 43625, wherein the resistance of the plant to Bremia lactucae is enhanced as compared with a L. sativa plant lacking said introgressed sequence, and wherein said introgressed sequence is located on chromosome 2 and comprises one or more of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker SL1831 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker SL1847 in SEQ ID NO:
- 6;
- c) a G genotype in the heterozygous or homozygous state for SNP marker SL1887 in SEQ ID NO:
- 11;
- and/or
- d) a C genotype in the heterozygous or homozygous state for SNP marker SL1883 in SEQ ID NO:
- 16.
Dependencies are inferred from the claim text.