Display substrate and method for preparing the same, and display device

A method for preparing a display substrate, comprising:

• forming an anode layer and a pixel definition layer on a base substrate, wherein the pixel definition layer defines a first subpixel area and a second subpixel area that are adjacent to each other, the pixel definition layer comprises a plurality of openings, the plurality of openings comprises a first opening and a second opening, the first subpixel area is located at a position corresponding to the first opening and the second subpixel area is located at a position corresponding to the second opening;
• sequentially forming a first hole transport layer and a first light emitting layer of the first subpixel on the anode layer in the first subpixel area;
• forming a spacer function layer for blocking hole transport between adjacent subpixels;
• and
• sequentially forming a second hole transport layer and a second light emitting layer of the second subpixel on the anode layer in the second subpixel area,
• wherein the spacer function layer is arranged between the first hole transport layer and the second hole transport layer and is at least arranged between the first opening and the second opening, the spacer function layer at least covers an edge area of the first hole transport layer of the first subpixel proximate to the second subpixel, so that the first hole transport layer and the second hole transport layer are not connected in a position arranged with the spacer function layer, a material of the spacer function layer is different from that of the first hole transport layer and the second hole transport layer, and
• wherein the spacer function layer extends to a pixel area of only one subpixel of the first subpixel and the second subpixel, and completely covers a light emitting area of the one subpixel, but does not cover the light emitting area of the other subpixel.