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US 12,744,230 B2Granted
Electron carrier, electron carrier production method, and electron transfer method
AssigneePanasonic Intellectual Property Management Co., Ltd.
InventorsShoko Kusama, Seiji Kojima
Life of this patent
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Abstract
An electron carrier includes a modified cyanobacterium in which at least: (i) a function of a protein involved in binding between an outer membrane and a cell wall of cyanobacterium is suppressed or lost; or (ii) a channel protein which improves protein permeability of the outer membrane is expressed. The modified cyanobacterium performs at least one of supplying electrons to an outside or taking in electrons from the outside.
Claims8
An electron carrier comprising:
- a modified cyanobacterium in which at least:
- (i) a function of a protein involved in binding between an outer membrane and a cell wall of cyanobacterium is suppressed;
- or
- (ii) a channel protein which improves protein permeability of the outer membrane is expressed, wherein
- the modified cyanobacterium performs at least one of supplying electrons to an outside or taking in electrons from the outside,
- wherein in (i), the protein involved in the binding between the outer membrane and the cell wall is at least one of a surface layer homology (SLH) domain-containing outer membrane protein or a cell wall-pyruvic acid modifying enzyme,
- wherein the SLH domain-containing outer membrane protein is:
- Slr1841 having the amino acid sequence of SEQ ID NO:
- 1;
- NIES970_09470 having the amino acid sequence of SEQ ID NO:
- 2;
- Anacy_3458 having the amino acid sequence of SEQ ID NO:
- 3;
- or
- a protein having an amino acid sequence that is at least 50 percent identical to the amino acid sequence of any one of the Slr1841, the NIES970_09470, and the Anacy_3458, and
- wherein the cell wall-pyruvic acid modifying enzyme is:
- Slr0688 having the amino acid sequence of SEQ ID NO:
- 4;
- Synpcc7942_1529 having the amino acid sequence of SEQ ID NO:
- 5;
- Anacy_1623 having the amino acid sequence of SEQ ID NO:
- 6;
- or
- a protein having an amino acid sequence that is at least 50 percent identical to the amino acid sequence of any one of the Slr0688, the Synpcc7942_1529, and the Anacy_1623.
21 more elements
An electron carrier comprising:
- a modified cyanobacterium in which at least:
- (i) a function of a protein involved in binding between an outer membrane and a cell wall of cyanobacterium is suppressed;
- or
- (ii) a channel protein which improves protein permeability of the outer membrane is expressed, wherein
- the modified cyanobacterium performs at least one of supplying electrons to an outside or taking in electrons from the outside,
- wherein in (i), a gene which causes expression of the protein involved in the binding between the outer membrane and the cell wall is deleted or inactivated,
- wherein the gene which causes expression of the protein involved in the binding between the outer membrane and the cell wall is at least one of a gene encoding an SLH domain-containing outer membrane protein or a gene encoding a cell wall-pyruvic acid modifying enzyme,
- wherein the gene encoding the SLH domain-containing outer membrane protein is:
- slr1841 having the nucleotide sequence of SEQ ID NO:
- 7;
- nies970_09470 having the nucleotide sequence of SEQ ID NO:
- 8;
- anacy_3458 having the nucleotide sequence of SEQ ID NO:
- 9;
- or
- a gene having a nucleotide sequence that is at least 50 percent identical to the nucleotide sequence of any one of the slr1841, the nies970_09470, and the anacy_3458, and
- wherein the gene encoding the cell wall-pyruvic acid modifying enzyme is:
- s1r0688 having the nucleotide sequence of SEQ ID NO:
- 10;
- synpcc7942_1529 having the nucleotide sequence of SEQ ID NO:
- 11;
- anacy_1623 having the nucleotide sequence of SEQ ID NO:
- 12;
- or
- a gene having a nucleotide sequence that is at least 50 percent identical to the nucleotide sequence of any one of the slr0688, the synpcc7942_1529, and the anacy_1623.
22 more elements
Dependencies are inferred from the claim text.