Methods for detecting phosphorylated alpha-synuclein
AssigneeProthena Biosciences Limited
InventorsRobin Barbour, Lynn R. Zieske, Sarah Hamren
Life of this patent
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Abstract
The invention provides methods of detecting alpha-synuclein using a capture antibody and a reporter antibody. The capture antibody binds preferentially to full-length alpha-synuclein phosphorylated at residue 129 (PS129 alpha-synuclein) over unphosphorylated full-length alpha-synuclein. The 11A5 antibody is an example of a suitable capture antibody. The reporter antibody binds to an epitope within residues 40-55 of alpha-synuclein. The 23E8 antibody is an example of such an antibody. Because only a small proportion of alpha-synuclein is phosphorylated high sensitivity of detection below picomolar is advantageous.
Claims15
A kit for detecting alpha-synuclein phosphorylated at serine 129 (PS129 alpha-synuclein), comprising:
- a capture antibody that preferentially binds to PS129 alpha-synuclein and a reporter antibody that specifically binds to an epitope within residues 40-55 of alpha-synuclein,
- wherein the capture antibody comprises the Kabat CDRs of 11A5, deposited under ATCC accession number PTA-8222, and the reporter antibody comprises the Kabat CDRs of 23E8, deposited under ATCC accession number PTA-122711.
Dependencies are inferred from the claim text.