US 12,742,788 B1Granted

Equine multiplex health information panel

AssigneeBoundless Diagnostics Inc

InventorsTrevor Morin, Josh Hayward, Kevin Grant

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Abstract

Disclosed are equine biomarker panels and methods for generating actionable health and training guidance from multiplex biochemical measurements. The panel is organized across physiologic axes: core inflammation (e.g., CRP, SAA, IL-6, S100A8/A9, IRF7), muscle/tissue injury and metabolic stress (e.g., lactate, CK, AST, LDH, myoglobin, ADAM-12), trauma/stress (e.g., HMGB1, haptoglobin, cortisol), and oxidative/joint/cartilage status (e.g., 8-isoprostane, COMP, MMP-3, MMP-8, ferritin). Quantitative values from an equine sample are combined into composite indices and/or ratiometric measures, optionally classified against subject-specific baselines and population references to produce readiness or risk categories. The method then outputs protocol-ready actions including training-load modification, rest or return-to-work guidance, re-test intervals, imaging/referral prompts, and selection of supplementation categories.

Claims2

Dependent claims

A multiplex biomarker panel, comprising:

a solid-phase substrate comprising a plurality of spatially discrete capture regions arranged as a multiplexed array;
and
an assay reagent set including a plurality of distinct affinity reagents, each affinity reagent immobilized at a respective one of the spatially discrete capture regions and each having specific binding affinity for a corresponding biomarker;
wherein the spatially discrete capture regions are arranged to enable simultaneous quantitative detection, in a single assay run, of a plurality of biomarkers from a single biological sample, the plurality of biomarkers comprising:
C-reactive protein (CRP), serum amyloid A (SAA), interleukin-6 (IL-6), calprotectin (S100A8/A9), interferon regulatory factor 7 (IRF7), lactate, creatine kinase (CK), aspartate transaminase (AST), lactate dehydrogenase (LDH), myoglobin, disintegrin and metalloproteinase domain-containing protein 12 (ADAM-12), high-mobility group box 1 (HMGB1), haptoglobin, cortisol, 8-isoprostane, cartilage oligomeric matrix protein (COMP), matrix-metallopeptidase-3 (MMP-3), matrix metallopeptidase-8 (MMP-8) and ferritin.

2 more elements

Dependencies are inferred from the claim text.