Information processing apparatus, information processing system, information processing method, and program

An information processing system comprising:

• an input unit that accepts an input of data regarding expression levels of a plurality of biomolecules to be used for analysis of a sample;
• a processing unit that:
• receives, from the input unit, an indication of the data;
• obtains an indication of a plurality of phosphors usable for analysis of the sample by a spectral flow cytometer an fluorescence spectra associated with the plurality of phosphors;
• generates expression level categories for the plurality of biomolecules based on expression levels in the sample,
• generates, based on the expression level categories, brightness categories for the plurality of phosphors based on brightness;
• associates the expression level categories with the brightness categories based on the expression levels and the brightness;
• generates, based on correlation information between the plurality of phosphors indicative of the fluorescence spectra associated with the plurality of phosphors, a combination list of phosphors to be used for the analysis of the sample by the spectral flow cytometer;
• outputs, for display by a display device, an indication of the phosphors of the combination list respectively allocated to the biomolecules of the plurality of biomolecules;
• receives, from the spectral flow cytometer, light intensity data of the plurality of biomolecules stained with the phosphors of the combination list which were respectively allocated;
• and
• deconvolutes the light intensity data based on the fluorescence spectra of the phosphors of the combination list;
• and
• the display device.

An information processing method comprising:

• receiving an indication of a plurality of biomolecules to be used for analysis of a sample and respective expression levels of the plurality of biomolecules;
• obtaining an indication of a plurality of phosphors usable for analysis of sample by a spectral flow cytometer and fluorescence spectra associated with the plurality of phosphors;
• generating expression level categories for the plurality of biomolecules based on expression levels in the sample;
• generating, based on the expression level categories, brightness categories for the plurality of phosphors based on brightness of the plurality of phosphors;
• associating the expression level categories with the brightness categories based on the expression levels and the brightness;
• generating, based on correlation information between the plurality of phosphors indicative of the fluorescence spectra associated with the plurality of phosphors, a combination list of phosphors to be used for the analysis of the sample by the spectral flow cytometer;
• allocating, based on the association between the expression level categories and the brightness categories, phosphors of the combination list to biomolecules of the plurality of biomolecules;
• outputting, for display by a display device, an indication of the phosphors of the combination list respectively allocated to the biomolecules of the plurality of biomolecules;
• receiving light intensity data of the plurality of biomolecules stained with the phosphors of the combination list which were respectively allocated;
• and
• deconvoluting the light intensity data based on the fluorescence spectra of the phosphors of the combination list.

A program causing an information processing apparatus to execute a method comprising:

• receiving an indication of a plurality of biomolecules to be used for analysis of a sample and respective expression levels of the plurality of biomolecules;
• obtaining an indication of a plurality of phosphors usable for analysis of sample by a spectral flow cytometer and fluorescence spectra associated with the plurality of phosphors;
• generating expression level categories for the plurality of biomolecules based on expression levels in the sample;
• generating, based on the expression level categories, brightness categories for the plurality of phosphors based on brightness of the plurality of phosphors;
• associating the expression level categories with the brightness categories based on the expression levels and the brightness;
• generating, based on correlation information between the plurality of phosphors indicative of the fluorescence spectra associated with the plurality of phosphors, a combination list of phosphors to be used for the analysis of the sample by the spectral flow cytometer;
• allocating, based on the association between the expression level categories and the brightness categories, phosphors of the combination list to biomolecules of the plurality of biomolecules;
• outputting, for display by a display device, an indication of the phosphors of the combination list respectively allocated to the biomolecules of the plurality of biomolecules;
• receiving light intensity data of the plurality of biomolecules stained with the phosphors of the combination list which were respectively allocated;
• and
• deconvoluting the light intensity data based on the fluorescence spectra of the phosphors of the combination list.