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US 12,742,772 B2Granted
DNA-based voltmeter for organelles
AssigneeThe University of Chicago
InventorsYamuna Krishnan, Aneesh Tazhe Veetil, Anand Saminathan
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Abstract
This disclosure relates to methods for determining membrane potential across membranes of organelles and cells. More particularly, this disclosure relates to nucleic add complexes comprising a voltage-sensing fluorophore conjugated to one single-stranded nucleic acid molecule and methods for using such nucleic acid complexes in determining the membrane potential.
Claims19
A nucleic acid complex comprising:
- a first single-stranded nucleic acid molecule comprising a voltage-sensing fluorophore crosslinked to the first strand;
- and
- a second single-stranded nucleic acid molecule that is partially or fully complementary to the first single-stranded molecule,
- wherein the nucleic acid complex further comprises a reference label conjugated to the first single-stranded nucleic acid molecule or the second single-stranded nucleic acid molecule and the reference label is capable of producing a signal.
1 more element
Dependencies are inferred from the claim text.