Method of acquiring liquid biological samples from an extracorporeal circuit for an extracorporeal blood purification treatment and related apparatus and system

An apparatus for acquisition of liquid biological samples from an extracorporeal circuit for subjecting a patient to an extracorporeal blood purification treatment, said apparatus comprising a first hydraulic machine, a second hydraulic machine, a third hydraulic machine, and comprising:

• I) a first collection chamber adapted to be in fluidic communication with a first sampling port of said extracorporeal circuit via said first hydraulic machine, said first sampling port being operatively connected to a first haematic line inputted to a haemodiafilter of said extracorporeal circuit;
• II) a second collection chamber adapted to be in fluidic communication with a second sampling port of said extracorporeal circuit via said second hydraulic machine, said second sampling port being operatively connected to a second haematic line outputted from said haemodiafilter;
• III) a third collection chamber adapted to be in fluidic communication with a third sampling port of said extracorporeal circuit via said third hydraulic machine, said third sampling port being operatively connected to an effluent line of said haemodiafilter,
• said apparatus being characterized in that said first hydraulic machine is adapted to fill said first collection chamber with a first biological sample (A), said second hydraulic machine is adapted to fill said second collection chamber with a second biological sample (B), and said third hydraulic machine is adapted to fill said third collection chamber with a third biological sample (C), said first hydraulic machine, said second hydraulic machine and said third hydraulic machine being activable at the same time and with a flow rate value.

An analysis apparatus comprising actuator means, sensor means, memory means, and processing means adapted to:

• receive a first collection chamber comprising a first biological sample (A), a second collection chamber comprising a second biological sample (B), and a third collection chamber comprising a third biological sample (C);
• subdivide each one of said first biological sample (A), second biological sample (B) and third biological sample (C), respectively, into at least a first share (A1, B1, C1), a second share (A2, B2, C2) and a third share (A3, B3, C3), said first share (A1, B1, C1) concerning a first solute having a first molecular mass value of 0 to 500 dalton, said second share (A2, B2, C2) concerning a second solute having a second molecular mass value of 501 to 5,000 dalton, and said third share (A3, B3, C3) concerning a third solute having a third molecular mass value of 5,001 to 100,000 dalton;
• determine, for each one of said first share (A1, B1, C1), second share (A2, B2, C2) and third share (A3, B3, C3), concentration values of the first solute (C_A1, C_B1, C_C1), concentration values of the second solute (C_A2, C_B2, C_C2) and concentration values of the third solute (C_A3, C_B3, C_C3);
• determine a first mass balance error (Err1), a second mass balance error (Err2) and a third mass balance error (Err3) on the basis of at least one of said concentration values of the first solute (C_A1, C_B1, C_C1), concentration values of the second solute (C_A2, C_B2, C_C2) and concentration values of the third solute (C_A3, C_B3, C_C3), said first mass balance error (Err1) concerning said first solute, said second mass balance error (Err2) concerning said second solute, and said third mass balance error (Err3) concerning said third solute;
• determine, for each one of said first solute, second solute and third solute, a clearance value (K1, K2, K3) and/or a sieving coefficient (H1, H2, H3) or an effluent saturation coefficient when each one of said first mass balance error (Err1), second mass balance error (Err2) and third mass balance error (Err3) is comprised between −10% and +10%.