Method for collecting cell types

A method for collecting cells, the method comprising:

• introducing a first cell load into a rotatable separation chamber of a reverse flow centrifugation device, wherein the first cell load comprises a first set of cells of interest and a first set of one or more other cell types;
• performing a first elutriation cycle on the first cell load to form a fluidized bed of the first set of cells of interest within the separation chamber while the first set of one or more other cell types are separated apart from the first set of cells of interest;
• introducing into the separation chamber a second cell load while the first set of cells of interest remains within the separation chamber, wherein the second cell load comprises a second set of cells of interest and a second set of one or more other cell types;
• and
• performing a second elutriation cycle on the second cell load to collect the second set of cells of interest on the fluidized bed of the first set of cells of interest in the separation chamber while the second set of one or more other cell types are separated apart from the first and second sets of cells of interest.