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US 12,742,216 B2Granted
Method for determining titer of RNA viral vectors
AssigneeAbelzeta Inc.
InventorsZefa Wang, Li Li, Hanning Zhang, Yilang Cao, Ting Han, Jishun Lu, Yi Hong, Li Zhang
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods for rapidly assaying the titer of an RNA viral vector such as a lentiviral vector. The method can be used to quickly evaluate the titer of intermediate products in various steps of a lentiviral vector production process.
Claims19
A method for determining a titer of an RNA viral vector in a sample, the method comprising:
- (a) providing a sample comprising an RNA viral vector and DNA molecules, or adding DNA molecules to a sample comprising an RNA viral vector, wherein both the RNA viral vector and the DNA molecules comprise a sequence element;
- (b) obtaining a first portion and a second portion from the sample;
- and
- (c) performing polymerase chain reaction (PCR) on the first portion to determine a copy number of the sequence element in the first portion (n1), and performing reverse transcription PCR (RT-PCR) on the second portion to determine a copy number of the sequence element in the second portion (n2), wherein an RNA copy number of the RNA viral vector in the sample is determined by a difference between n1 and n2 which is n2−n1.
1 more element
Dependencies are inferred from the claim text.