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US 12,742,211 B2Granted
Buffer compositions for reducing aggregation
AssigneeAugusta SpinCo Corporation
InventorsJean-Sebastien Cote, Marie-Christine Fortin, Vincent Blanchette, Marie-Helene Tremblay, Sebastien Morasse, Sophie Guay, Sebastien Simard
Life of this patent
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Abstract
Disclosed include compositions, kits and methods for nucleic acid-based detection and identification of vaginal disorders, for example, vulvovaginal candidiasis, trichomoniasis and/or bacterial vaginosis. The compositions, kits and methods can be used for obtaining robust diagnostic testing performance against interfering substances, for example, gels present in clinical vaginal-swab samples.
Claims18
A buffer composition, comprising:
- (a) 50 mM to 150 mM acetic acid and 350 mM to 450 mM sodium acetate,
- 1 mM to 20 mM ethylenediaminetetraacetic acid (EDTA),
- 0.1% w/w to 3% w/w non-ionic surfactant,
- 100 mM to 300 mM of a divalent salt selected from the group consisting of calcium salts, magnesium salts, and combinations thereof, and
- a pH of 4.0 to 6.0;
- or
- (b) 1 mM to 20 mM Tris-HCl,
- 0.1% w/w to 3% w/w non-ionic surfactant,
- 1 mM to 30 mM tris(2-carboxyethyl)phosphine (TCEP),
- 50 mM to 150 mM of a divalent salt selected from the group consisting of calcium salts, magnesium salts, and combinations thereof, and
- a pH of 2.1 to 3.0.
8 more elements
A buffer composition comprising:
- 10 mM Tris-HCl,
- 1% polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether,
- 15 mM TCEP,
- 50-150 mM CaCl2, and
- a pH of 2.4±0.2.
2 more elements
A buffer composition comprising:
- 50 mM to 150 mM acetic acid,
- 350 mM to 450 mM sodium acetate,
- 1 mM to 20 mM EDTA,
- 0.1% w/w to 3% w/w non-ionic surfactant,
- 100 mM to 300 mM CaCl2,
- 0.01% to 1% chloromethylisothiazolinone and methylisothiazolinone, and
- a pH of 4.0 to 6.0.
4 more elements
Dependencies are inferred from the claim text.