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US 12,742,203 B2Granted
Rotatable discs and systems and methods for sample analysis
AssigneeAutonomous Medical Devices Inc.
InventorsRegis Peytavi, Horacio Kido
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Abstract
The present invention describes a method and device enabling sample processing, fast polymerase chain reaction amplification, and real-time reading of a large number of targets simultaneously while using a volume of reaction compatible with molecular assays. The present invention can be part of a cartridge allowing sample to answer nucleic acid analytic system (NAAT) or can be used as a stand-alone device.
Claims30
A method of detecting the presence or absence of a target nucleic acid in a sample, the method comprising:
- (a) providing a rotatable PCR disc comprising a closed loading chamber, a plurality of reaction chambers, a channel connecting the closed loading chamber to the plurality of reaction chambers, and an exit valve connecting the closed loading chamber to the channel, wherein the closed loading chamber contains the sample;
- (b) heating the sample in the closed loading chamber in a hyperbaric condition to a temperature above 100° C. to produce a heat-treated sample;
- (c) opening the exit valve, thereby opening the closed loading chamber, and rotating the rotatable PCR disc such that the heat-treated sample flows to the plurality of reaction chambers via the channel;
- and
- (d) amplifying the target nucleic acid, if present, in the heat-treated sample in the plurality of reaction chambers.
2 more elements
Dependencies are inferred from the claim text.