Method for extraction and multi-scenario utilization of flaxseed protein-polysaccharide natural mixture

A method for extraction and multi-scenario utilization of a flaxseed protein-polysaccharide natural mixture, specifically comprising the following steps:

• (1) raw material pretreatment
• firstly crushing, sieving and degreasing flaxseed meal to obtain flaxseed degreased meal powder;
• and then dissolving the flaxseed degreased meal powder in water and stirring to obtain flaxseed degreased meal powder rinsing solution;
• (2) extraction of flaxseed protein-polysaccharide natural mixture I
• firstly conducting ultrasonic treatment for the flaxseed degreased meal powder rinsing solution, or firstly conducting microwave treatment for the flaxseed degreased meal powder rinsing solution, or firstly adding a bio-enzyme into the flaxseed degreased meal powder rinsing solution and stirring in a water bath, wherein the bio-enzyme is pectase, cellulase or hemicellulase;
• then centrifuging, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture I;
• (3) utilization of the flaxseed protein-polysaccharide natural mixture I
• firstly, dissolving the flaxseed protein-polysaccharide natural mixture I prepared by ultrasonic treatment in the step (2), gum Arabic and maltodextrin in water and hydrating overnight;
• then adding oil containing n-3 polyunsaturated fatty acid, shearing, introducing a high-pressure microfluidization, adding food grade silica powder and shaking up to obtain emulsion containing n-3 polyunsaturated fatty acid;
• and finally, spray-drying the emulsion to obtain powdered oil containing n-3 polyunsaturated fatty acid, wherein a pressure of the high-pressure microfluidization is 750 bar;
• or, firstly dissolving the flaxseed protein-polysaccharide natural mixture I prepared by microwave treatment in the step (2) in water and adjusting pH to acidity;
• then heating in a water bath and whipping;
• and finally, treating in an ice bath to room temperature to obtain gel foam;
• or, using the flaxseed protein-polysaccharide natural mixture I prepared by the bio-enzyme in the step (2) as a protein ingredient for preparing a low-turbidity plant protein drink;
• (4) extraction of flaxseed protein-polysaccharide natural mixture II
• firstly, redissolving a precipitate obtained after centrifugation in the step (2) to same volume as the flaxseed degreased meal powder rinsing solution to obtain a redissolution solution, adjusting pH of the redissolution solution to alkalinity and conducting magnetic stirring to obtain an alkali extraction solution of the flaxseed degreased meal powder;
• then, conducting ultrasonic treatment for the alkali extraction solution of the flaxseed degreased meal powder, centrifuging, taking supernatant and freeze-drying to obtain the flaxseed protein-polysaccharide natural mixture II;
• (5) utilization of the flaxseed protein-polysaccharide natural mixture II
• firstly dissolving the flaxseed protein-polysaccharide natural mixture II in water, then adding the oil containing n-3 polyunsaturated fatty acid, and after shearing, introducing a high-pressure microfluidization to obtain low oil phase nanoemulsion, wherein a pressure of the high-pressure microfluidization is 500 bar;
• or, firstly dissolving the flaxseed protein-polysaccharide natural mixture II and sucrose in water, adding coconut oil, shearing to obtain macroemulsion, introducing the high-pressure microfluidization to obtain miniemulsion, then adding the flaxseed protein-polysaccharide natural mixture I prepared by microwave treatment in the step (2) for magnetic stirring, aging, adjusting pH to acidity, and whipping in an ice bath to obtain foamed emulsion;
• or, mixing and crushing the flaxseed protein-polysaccharide natural mixture II, pea protein isolate, hemp seed protein, soybean protein isolate, perilla protein, perilla peptide, microcapsule powder containing n-3 polyunsaturated fatty acid, medium chain triglycerides microcapsule powder, psyllium husk powder, maltodextrin, oat flour, flaxseed powder, resistant dextrin, chitosan oligosaccharide, yacon, inulin, fructo oligosaccharide, erythritol, konjaku flour and stevioside to obtain a meal replacement milkshake.