Production of fructose from oligo-/ and/or polysaccharides

A method for converting at least one oligosaccharide and/or polysaccharide into fructose comprising the steps of:

• a) adding to a composition comprising water, phosphate, at least one oligosaccharide and/or polysaccharide, at least six enzymes, and
• b) subsequently enzymatically converting the at least one oligosaccharide and/or polysaccharide to fructose in the presence of the at least six enzymes,
• wherein in step a) at least one additional saccharide is added, whereby the at least one additional saccharide is selected from the group consisting of tetradecasaccharides, tridecasaccharides, dodecasaccharides, undecasaccharides, decasaccharides, nonasaccharides, octasaccharides, heptasaccharides, hexasaccharides, pentasaccharides, tetrasaccharides, trisaccharides, and/or disaccharides;
• wherein in step a) the at least six enzymes that are added to the composition include at least one transferase, at least one phosphorylase, at least one mutase, at least one isomerase, and at least one hydrolase;
• wherein at least one enzyme of the at least six enzymes in step a) is a phosphatase;
• wherein the at least one oligosaccharide and/or polysaccharide is selected from glucose-based oligosaccharide and/or polysaccharides;
• and
• wherein the phosphatase comprises an amino acid sequence that is at least 99% identical with the sequence according to SEQ ID NO:
• 41.