Application of transport carrier gene which improves L-tryptophan production efficiency in Escherichia coli
AssigneesNingxia Eppen Biotech Co., Ltd, Tianjin University of Science and Technology
InventorsXixian Xie, Bo Xiong, Chunguang Zhao, Xiaowei Guo, Jiaxuan Men, Aiying Wei
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Abstract
A transport protein coding gene, and a method for efficient production of L-tryptophan by a strain containing the gene. Specifically, by heterologous expression of ywkB gene from Bacillus subtilis on the genome of Escherichia coli, L-tryptophan production efficiency of the strain can be improved. Performing shake flask fermentation with the strain can accumulate 15.2 g/L of L-tryptophan within 24 h, which is 35% higher than a control strain.
Claims4
A method for constructing a genetically engineered bacterial strain comprising a step of integrating a ywkB gene into the yeep pseudogene locus of an Escherichia coli (E. coli) host strain which produces L-tryptophan,
- wherein the ywkB gene encodes the amino acid sequence of SEQ ID NO:
- 2, thereby producing a genetically-engineered bacterial strain which produces a higher yield of L-tryptophan than the E. coli host strain.
Dependencies are inferred from the claim text.