Method for producing genetically edited birds having resistance to avian influenza viruses
AssigneeSeoul National University R&Db Foundation
InventorsJae Yong Han, Young Hyun Park, Jeong Yong Suh, Jeong Mook Lim
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Abstract
Provided is a method of producing a genome-edited bird having resistance to avian influenza viruses. A method according to an aspect may enable precise inhibition of interaction between virus proteins while maintaining the original function of an ANP32A gene in a host by substituting only key amino acids of the ANP32A gene. When the method is used, by including cell lines having resistance to avian influenza viruses, new poultry and bird breeds that may not pose any biological safety issues may be efficiently developed. Thus, it is expected that the potential for industrial application is high.
Claims16
A recombinant vector comprising a polynucleotide encoding a guide RNA (gRNA), wherein the guide RNA (gRNA) targets nucleotide sequences encoding amino acid residues of Asp149, Asp152, Asp182, and Asp185 in an Acidic Nuclear Phosphoprotein 32 Family Member A (ANP32A) gene to induce substitutions of the amino acid residues with Tyr149, His152, Tyr182 and His185, respectively.
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