Modulating alkaloid profiles in Nicotiana tabacum
AssigneePhilip Morris Products S.A.
InventorsLucien Bovet, Aurore Hilfiker, Kacper Piotr Kaminski
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Abstract
A non-naturally occurring or transgenic Nicotiana tabacum plant cell encompassing a heterologous polynucleotide that encodes arginine decarboxylase (ADC) and a method for producing such a cell are provided. Transgenic Nicotiana tabacum plants, plant parts, and plant tissues including these cells and plant materials and biomass produced by these cells and plants cells forming such a plant are provided. Materials produced by this plant, including plant leaves, flowers, seeds, extracts, and other plant biomass obtained from this plant including materials that have altered alkaloid levels are disclosed. Also provided are smoking articles including various tobacco products containing these materials.
Claims18
A transgenic Nicotiana tabacum plant cell comprising a heterologous polynucleotide sequence that encodes a heterologous polypeptide sequence selected from:
- a) a polypeptide sequence having at least 97% sequence identity to SEQ ID NO:
- 2, wherein the amino acid at position 31 is G, 40 is L, 63 is C, 296 is E, 355 is S, 417 is S, 429 is Y, 438 is S, 652 is M, 658 is D, 661 is M, 700 is D, 707 is I, and 730 is V;
- and
- b) a polypeptide sequence having at least 97.5% sequence identity to SEQ ID NO:
- 4, wherein the amino acid at position 12 is A, 37 is I, 87 is M, 151 is F, 317 is V, 378 is E, 429 is V, 530 is V, and 680 is V;
- wherein the heterologous polynucleotide sequence is operably linked to a promotor directing expression of the heterologous polypeptide sequence.
A transgenic Nicotiana tabacum plant cell comprising a heterologous polynucleotide sequence that encodes a heterologous polypeptide sequence having at least at least 97.5% sequence identity to SEQ ID NO:
- 6, wherein the amino acid at position 90 is M, 203 is F, 296 is E, 342 is V, 380 is Q, 441 is G, 619 is N, 628 is S, 663 is D, 664 is H, 680 is L, 698 is S, and 715 is S;
- wherein the heterologous polynucleotide sequence is operably linked to a promotor directing expression of the heterologous polypeptide sequence.
Dependencies are inferred from the claim text.