Anthocyanin biosynthesis in carrot plants

A DNA sequence consisting of:

• (a) a first transcription factor gene DcMYB90 (SEQ ID NO:1) operably linked to a heterologous promoter, and a second transcription factor gene DcEGL1 (SEQ ID NO:2) operably linked to a heterologous promoter;
• or
• (b) a first transcription factor gene having a sequence with at least 95% identity to the sequence of SEQ ID NO:1 operably lined to a heterologous promoter, and the second transcription factor gene have a sequence with at least 95% identity to the sequence of SEQ ID NO:
• 2, operably linked to a heterologous promoter;
• wherein the first transcription factor gene DcMYB90 (SEQ ID NO. 1) encodes the transcription factor protein of SEQ ID NO. 5, and the transcription factor gene DcEGL1 (SEQ ID NO. 2) encodes the transcription factor protein of SEQ ID NO. 6, and wherein the co-expression of the proteins increases the expression of sinapic acid glucosyltransferase (USAGT) having the sequence of SEQ ID NO:4, and wherein the heterologous promoter achieves an increase in expression of more than 30% in comparison to the expression of the corresponding transcription factor under its natural promoter.

A method of producing a carrot plant comprising transforming an orange carrot plant with:

• a. (a) at least one DNA sequence consisting of the transcription factor gene DcMYB90 (SEQ ID NO:1) operably linked to a heterologous promoter and at least one heterologous DNA sequence consisting of the transcription factor gene DcEGL1 (SEQ ID NO:2) operably linked to a heterologous promoter;
• of
• b. at least one DNA sequence having at least 95% identity to the sequence of SEQ ID NO:1, and at least one DNA sequence having at least 95% identity to the sequence of SEQ ID NO:2 operably linked to a heterologous promoter;
• wherein the transcription factor gene DcMYB90 (SEQ ID NO:1) encodes the transcription factor protein of SEQ ID NO;
• 5, and the transcription factor gene DcEGL1 (SEQ ID NO:2) encodes the transcription factor protein of SEQ ID NO:
• 6, and wherein the co-expression of the proteins
• wherein the heterologous DNA sequence increases the expression of sinapic acid glucosyltransferase (USAGT) having the sequence of SEQ ID NO:4.