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US 12,742,167 B2Granted
Systems and methods for biomolecule retention
AssigneeNautilus Subsidiary, Inc.
InventorsTorri Elise Rinker, Christina Inman, Parag Mallick, Tural Aksel, Stephen Hendricks, Pierre Indermuhle, Hongji Qian, Steven Tan, Elvis Ikwa, Pengyu Hao, Sadie Ingle
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Abstract
Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.
Claims26
A system, comprising:
- a) a solid support comprising a plurality of sites, wherein a plurality of nucleic acid particles is attached to the plurality of sites, wherein each site of the plurality of sites is attached to only one nucleic acid particle of the plurality of nucleic acid particles, wherein a plurality of polypeptides is attached to the plurality of nucleic acid particle, wherein each nucleic acid particle of the plurality of nucleic acid particles is attached to only one polypeptide of the plurality of polypeptides, wherein each nucleic acid particle of the plurality of nucleic acid particles comprises a first double-stranded region that is attached to the solid support and a second double-stranded region that is oriented substantially orthogonal to the surface of the solid support, wherein only one polypeptide of the plurality of polypeptides is attached to the second double-stranded region, and wherein each site of the plurality of sites is optically resolvable from any other site of the plurality of sites;
- b) a first fluidic medium comprising a first plurality of binding reagents, wherein binding reagents of the first plurality of binding reagents are configured to provide a first optical signal, and wherein the first fluidic medium is deliverable to the solid support;
- c) a second fluidic medium comprising a second plurality of binding reagents, wherein binding reagents of the second plurality of binding reagents are configured to provide a second optical signal, wherein the second optical signal is distinguishable from the first optical signal, and wherein the second fluidic medium is deliverable to the solid support;
- and
- d) an optical detector, wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a first optical signal from a binding reagent of the first plurality of binding reagents, and wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a second optical signal from a binding reagent of the second plurality of binding reagents.
2 more elements
A system, comprising:
- a) a solid support comprising a plurality of immobilized nucleic acid particles, wherein the plurality of immobilized nucleic acid particles is attached to a plurality of polypeptides, wherein the plurality of polypeptides comprises a first polypeptide species and a second polypeptide species, wherein the first polypeptide species and the second polypeptide species have a dynamic range of at least 104 with respect to each other, wherein each immobilized nucleic acid particle of the plurality of nucleic acid particles comprises a first double-stranded region that is attached to the solid support and a second double-stranded region that is oriented substantially orthogonal to the surface of the solid support, and wherein only one polypeptide of the plurality of polypeptides is attached to the second double-stranded region;
- b) a first fluidic medium comprising a first plurality of binding reagents, wherein binding reagents of the first plurality of binding reagents are configured to provide a first optical signal, and wherein the first fluidic medium is deliverable to the solid support;
- c) a second fluidic medium comprising a second plurality of binding reagents, wherein binding reagents of the second plurality of binding reagents are configured to provide a second optical signal, wherein the second optical signal is distinguishable from the first optical signal, and wherein the second fluidic medium is deliverable to the solid support;
- and
- d) an optical detector, wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a first optical signal from a binding reagent of the first plurality of binding reagents, and wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a second optical signal from a binding reagent of the second plurality of binding reagents.
2 more elements
A system, comprising:
- a) a solid support comprising a plurality of sites, wherein a plurality of nucleic acid particles is attached to the plurality of sites, wherein each site of the plurality of sites is attached to only one nucleic acid particle of the plurality of nucleic acid particles, wherein a plurality of polypeptides is attached to the plurality of nucleic acid particles, wherein each nucleic acid particle is attached to only one polypeptide of the plurality of polypeptides, wherein the plurality of polypeptides comprises a first proteoform of a protein and a second proteoform of the protein, wherein each nucleic acid particle of the plurality of nucleic acid particles comprises a first double-stranded region that is attached to the solid support and a second double-stranded region that is oriented substantially orthogonal to the surface of the solid support, wherein only one polypeptide of the plurality of polypeptides is attached to the second double-stranded region;
- b) a first fluidic medium comprising a first plurality of binding reagents, wherein binding reagents of the first plurality of binding reagents have a binding specificity for the first proteoform of the protein, wherein binding reagents of the first plurality of binding reagents are configured to provide a first optical signal, and wherein the first fluidic medium is deliverable to the solid support;
- c) a second fluidic medium comprising a second plurality of binding reagents, wherein binding reagents of the second plurality of binding reagents have a binding specificity for the second proteoform of the protein, wherein binding reagents of the second plurality of binding reagents are configured to provide a second optical signal, wherein the second optical signal is distinguishable from the first optical signal, and wherein the second fluidic medium is deliverable to the solid support;
- and
- d) an optical detector, wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a first optical signal from a binding reagent of the first plurality of binding reagents, and wherein the optical detector is configured to detect at each individual site of the plurality of sites a presence or an absence of a second optical signal from a binding reagent of the second plurality of binding reagents.
2 more elements
Dependencies are inferred from the claim text.