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US 12,742,156 B2Granted
Methods and systems for transportation and culture of buoyant tissue
AssigneeKeliomics, Inc.
InventorsFrank Ho Pak Lau, Jason Bourgeois
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Abstract
Apparatuses, systems, and methods are provided for culturing a buoyant target tissue using a sandwich construct. Embodiments include preparing a buoyant target tissue in a sandwich construct for shipment, and preparing a sandwich construct comprising live tumor tissue for evaluation of candidate therapies for treating the tumor. The buoyant target tissue may include various cell types including cancer cells and tumors.
Claims7
A method for evaluating a candidate treatment for a breast cancer tumor in an individual, the method comprising:
- isolating a population of adipose-derived stromal cells (ASCs) that form a population of support cells from a first sample of healthy breast adipose tissue;
- culturing the population of support cells to form two layers of support cells;
- preparing a target tissue by mixing (i) a second sample of healthy buoyant tissue, wherein the healthy buoyant tissue is breast adipose tissue that has not been digested with collagenase, and (ii) a third sample of breast cancer tumor tissue;
- assembling a sandwich construct in a culture vessel and culturing the sandwich construct in the culture vessel, wherein the sandwich construct comprises a bi-layer construct in which the two layers of support cells sandwich the target tissue;
- exposing the sandwich construct to the candidate treatment;
- and
- evaluating the change in the status of the breast cancer tumor tissue in the target tissue in response to the candidate treatment.
4 more elements
Dependencies are inferred from the claim text.