Avian enteroids
AssigneeThe University Court of the University of Edinburgh
InventorsApolonia Vervelde, Esther Jane Nash
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Abstract
There is provided an in vitro three dimensional cell construct for use as a model of the avian intestine derived from avian intestinal tissue comprising avian cells organised into intestinal villi and crypts. Suitably the construct comprises an exterior surface that mimics the apical surface of a chicken intestine. Also provided are methods of making the cell construct and use of the construct as an in vitro intestinal model system to examine an agent including, but not limited to a microbe, a vaccine, a pharmaceutical, a feed additive, a toxin, a pre-biotic, post-biotic, pre pro post biotic, therapeutic, a cell, gene construct, protein, immune-modulator, an intestinal effector agent, a candidate intestinal effector agent, cell signalling inhibitor, or cell signalling activator.
Claims5
An in vitro multilobulated three dimensional avian enteroid comprising multiple differentiated intestinal cell types of both epithelial and mesenchymal lineages, for use as a model of the avian intestine, wherein the enteroid comprises:
- a. an inner core comprising lamina propria leukocytes,
- b. Intraepithelial leukocytes, and
- c. an exterior comprising an apical epithelial cell surface comprising differentiated intestinal epithelial cells bound by cell-cell junctions, with a morphology comprising villi and crypt structures.
Dependencies are inferred from the claim text.