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US 12,742,154 B2Granted
Methods and compositions for cell culture on heterogeneous scaffolds
AssigneeThe Regents of the University of California
InventorsAmy Rowat, Nora Stephanie Kawecki, Siobhan Ariel Braybrook, Sam Norris
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Abstract
Embodiments of the invention describe a cultured meat or food product with more desirable flavor and texture, as well as methods and compositions for producing the same. Certain embodiments are directed to methods of producing a stiffer, structured surfaces or scaffolds that mimic the extracellular matrix (ECM) and support the growth of myotubes that are interspersed with a scaffold component supporting fat cells (adipocytes) in vitro.
Claims21
A cultured food product comprising a mixture of:
- a first microcarrier scaffold containing myocytes;
- and
- a second microcarrier scaffold containing adipocytes,
- and wherein myocytes and adipocytes in the cultured food product are at a ratio between 20:1 to 2:1 and wherein the first microcarrier scaffold has a nanofiber component that is an aligned nanofiber component comprising aligned nanofibers.
1 more element
Dependencies are inferred from the claim text.