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US 12,742,151 B2Granted
Production method for microglia
AssigneesRicoh Company, Ltd., Elixirgen Scientific, Inc.
InventorsMasayuki Yumoto, Yudai Kawashima, Hikaru Watanabe, Toshihiko Hosoya, Minoru Ko
Life of this patent
Estimating the term…
Abstract
There is provided a production method for microglia, including a step of introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a transcription factor that increases expression of a Complement C3 (C3) gene. In the production method, the transcription factor is selected from the group consisting of SPI1, CEBPA, PTAFR, FLI1, MEF2C, EGR2, RUNX1, TNF, CEBPB, IKZF1, KLF6, NFIC, ELF4, PAX8, PRDM1, MEF2A, and NR4A3.
Claims2
A method of producing microglia, comprising:
- introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a combination of transcription factors that increases expression of a Complement C3 (C3) gene, wherein the combination of transcription factors is selected from the group consisting of BIN, CIN, BN, BKN, BEN, BLN, IKN and KLN, wherein B is CEBPA, C is PTAFR, E is MEF2C, I is CEBPB, K is KLF6, L is NFIC, and N is PAX8.
Dependencies are inferred from the claim text.