Systems, devices and methods for engineered tissue construct transport and containment

An engineered tissue construct (ETC) transport containment method comprising:

• aseptically cultivating an ETC within a bioreactor;
• de-cellularizing the ETC within the bioreactor, the de-cellularized ETC comprising extracellular matrix;
• storing the bioreactor within a transport package via an opening in the transport package;
• sealing the opening by applying a sealing membrane across the opening, the sealing membrane including a plurality of pores configured to pass gaseous and/or vaporous molecules of a predetermined size into and out of the interior of the transport package via the opening;
• placing the sealed transport package in a chamber;
• performing a sterilization process on the transport package within the chamber, the sterilization process comprising:
• providing an airflow to the chamber at an airflow rate;
• maintaining the chamber at a chamber pressure below atmospheric pressure;
• dehumidifying the airflow so as to dehumidify the chamber, the dehumidified airflow being provided to the chamber for a dehumidification time;
• after dehumidification, conditioning the airflow by injecting a conditioning amount of a sterilizing substance comprising the gaseous and/or vaporous molecules into the airflow for a conditioning time;
• after injecting the conditioning amount of the sterilizing substance, injecting a decontamination amount of the sterilizing substance into the airflow to establish and/or maintain a decontamination airflow entrained with the sterilizing substance within the chamber;
• and
• exposing, within the chamber, the sealed transport package to the decontamination airflow for a decontamination time so as to decontaminate the interior of the transport package and the exterior of the bioreactor contained therein.