Devices, systems, and methods relating to pressurized perfusion-enabled bio-reactors

A positive pressure perfusion-enabled bioreactor, comprising:

• a bioreactor having a central chamber bound by a high pressure side and a low pressure side and one or more walls;
• and
• an interlocking cap and frame in the central chamber,
• wherein the cap comprises a plurality of retrieval features arranged around a central cap aperture and extending toward an opening at the high pressure side of the bioreactor;
• wherein the frame comprises a plurality of height offsets arranged around a central frame aperture and extending toward the low pressure side of the bioreactor in a direction opposite the retrieval features;
• wherein the cap and frame are configured to interlock through an interference fit, forming an interlocked cap and frame, thereby aligning and forming a sample chamber in between the central frame aperture and the central cap aperture;
• and
• one or more membranes spanning the central frame aperture, the central cap aperture, or both.