US 12,742,140 B2Granted

Culturing system and method for culturing cells

AssigneesI Peace, Inc., Koji Tanabe

InventorsKoji Tanabe, Kenta Suto, Ryoji Hiraide

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Abstract

The present invention provides a culturing system and method for culturing cells that can culture cells in parallel. The culturing system comprises a cell storage 10 configured to store cells, a plurality of culture apparatuses 20, and a distribution flow path 30 configured to distribute the cells from the cell storage 10 to the culture apparatuses 20, in which each of the culture apparatuses 20 comprises a plurality of culture vessels 21, a supply flow path 22 configured to selectively supply a fluid from the distribution flow path 30 to the culture vessels 21, and a connection flow path 23 configured to connect the culture vessels 21; the culture vessels 21 are connected in series with the connection flow path 23 in each of the culture apparatuses 20; and the supply flow path 22 is configured to selectively supply the fluid to the culture vessels so that the cells are sequentially transferred among the culture vessels 21 through the connection flow path 23 in each of the culture apparatuses 20.

Claims9

Dependent claims

A culture system comprising:

a cell storage configured to store cells;
a plurality of culture apparatuses;
and
a distribution flow path configured to distribute the cells from the cell storage to the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses comprises:
a plurality of culture vessels;
a supply flow path configured to selectively supply a fluid from the distribution flow path to the plurality of culture vessels;
and
a connection flow path configured to connect the plurality of culture vessels,
wherein the plurality of culture vessels is connected in series with the connection flow path in each of the plurality of culture apparatuses, and
wherein the supply flow path is configured to selectively supply the fluid to the plurality of culture vessels so that the cells are sequentially transferred among the plurality of culture vessels through the connection flow path in each of the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses further comprises:
a plurality of supply valves provided in the supply flow path;
a discharge flow path configured to selectively discharge a fluid from the plurality of culture vessels;
and
a plurality of discharge valves provided in the discharge flow path,
wherein the plurality of supply valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein the plurality of discharge valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein one of the plurality of supply valves corresponding to one of the plurality of culture vessels that is to be provided with the cells from the cell storage is opened, and
wherein one of the plurality of discharge valves corresponding to one of the plurality of culture vessels that is to be provided with the cells from the cell storage is opened.

17 more elements

A culture system comprising:

a cell storage configured to store cells;
a plurality of culture apparatuses;
and
a distribution flow path configured to distribute the cells from the cell storage to the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses comprises:
a plurality of culture vessels;
a supply flow path configured to selectively supply a fluid from the distribution flow path to the plurality of culture vessels;
and
a connection flow path configured to connect the plurality of culture vessels,
wherein the plurality of culture vessels is connected in series with the connection flow path in each of the plurality of culture apparatuses, and
wherein the supply flow path is configured to selectively supply the fluid to the plurality of culture vessels so that the cells are sequentially transferred among the plurality of culture vessels through the connection flow path in each of the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses further comprises:
a plurality of supply valves provided in the supply flow path;
a discharge flow path configured to selectively discharge a fluid from the plurality of culture vessels;
and
a plurality of discharge valves provided in the discharge flow path,
wherein the plurality of supply valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein the plurality of discharge valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein one of the plurality of supply valves corresponding to one of the plurality of culture vessels that is to be provided with the fluid is opened, and
wherein one of the plurality of discharge valves corresponding to one of the plurality of culture vessels that is to be provided with the fluid is opened.

17 more elements

A culture system comprising:

a cell storage configured to store cells;
a plurality of culture apparatuses;
and
a distribution flow path configured to distribute the cells from the cell storage to the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses comprises:
a plurality of culture vessels;
a supply flow path configured to selectively supply a fluid from the distribution flow path to the plurality of culture vessels;
and
a connection flow path configured to connect the plurality of culture vessels,
wherein the plurality of culture vessels is connected in series with the connection flow path in each of the plurality of culture apparatuses, and
wherein the supply flow path is configured to selectively supply the fluid to the plurality of culture vessels so that the cells are sequentially transferred among the plurality of culture vessels through the connection flow path in each of the plurality of culture apparatuses,
wherein each of the plurality of culture apparatuses further comprises:
a plurality of supply valves provided in the supply flow path;
a plurality of connection valves provided in the connection flow path;
a discharge flow path configured to selectively discharge a fluid from the plurality of culture vessels;
and
a plurality of discharge valves provided in the discharge flow path,
wherein the plurality of supply valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein the connection valves are provided between the culture vessels in each of the plurality of culture apparatuses,
wherein the plurality of discharge valves correspond to the plurality of culture vessels, respectively, in each of the plurality of culture apparatuses,
wherein one of the plurality of supply valves corresponding to one of the plurality of culture vessels where the cells are disposed is opened, and
wherein the connection valve between the culture vessel where the cells are disposed and a culture vessel adjacent to the culture vessel where the cells are disposed is opened, and
wherein one of the plurality of discharge valves corresponding to the adjacent culture vessel is opened.

20 more elements

Dependencies are inferred from the claim text.