Stable reactive compositions for bioconjugation, probes, and protein labeling
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsJohn C. Jewett, Garrett Davis, Holly Sofka
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Abstract
Modular Meldrum's acid amine-reactive Michael acceptor (MaMa)—based molecules that are stable probes for high pH environments and bioconjugations. The molecules of the present invention can selectively label and protect lysine residues. In certain embodiments, the molecules can selectively react with lysine at particular pH levels. The probes of the present invention may be used to label proteins in a fluorescent manner, for purification, imaging, or general protein modifications, however the present invention is not limited to the aforementioned applications.
Claims3
A kit comprising:
- a composition according to Formula A in aqueous solution;
- and a set of instructions or access thereto for reacting the composition according to Formula A with an amine to produce a stable double amine adduct;
- wherein R is an azide, or a tetrazine;
- and wherein R′ is —CH3 or an alkyl group.
Dependencies are inferred from the claim text.