circCDK13-enriched engineered small extracellular vesicle (E-sEV), and preparation method and use thereof

Abstract

The present disclosure provides a circCDK13-enriched engineered small extracellular vesicle (E-sEV), and a preparation method and use thereof, and belongs to the technical field of biomedicine. The present disclosure provides a preparation method of the circCDK13-enriched E-sEV. In the present disclosure, human placenta-derived mesenchymal stem cells (hP-MSCs) are infected with a vector overexpressing circCDK13, and a resulting cell supernatant is collected to obtain a small extracellular vesicle (sEV) overexpressing the circCDK13, which is used for wound healing in diabetes mellitus (DM). The E-sEV shows a therapeutic effect on DM wounds that is significantly better than that of natural small extracellular vesicles (N-sEVs) secreted by the hP-MSCs. This product not only exhibits advantages in healing speed, but also has a greater application potential in stimulating skin appendage regeneration and improving the quality of wound healing.

Dependencies are inferred from the claim text.