Tissue culture method and propagation method of Cathaya argyrophylla

A tissue culture method of Cathaya argyrophylla, comprising the following steps:

• inoculating an explant of the Cathaya argyrophylla into an induction medium to allow induction culture to obtain an adventitious bud of the Cathaya argyrophylla;
• wherein the explant comprises a zygotic embryo;
• the explant is derived from Cathaya argyrophylla in Jinxiu Yao Autonomous County of Guangxi or Huaping Town in Guilin;
• the induction medium comprises a basic medium and 1.5 mg/L of 6-benzyladenine (6-BA);
• and the basic medium of the induction culture is selected from the group consisting of a DCR medium and a P6 medium;
• inoculating the adventitious bud of the Cathaya argyrophylla into a proliferation medium to allow proliferation culture to obtain a proliferated adventitious bud;
• wherein
• when the explant is derived from the Cathaya argyrophylla in the Jinxiu Yao Autonomous County of Guangxi, the proliferation medium uses the DCR medium as a basic medium, and further comprises 0.5 mg/L of the 6-BA and 0.2 mg/L of 1-naphthylacetic acid (NAA);
• alternatively, the proliferation medium uses the DCR medium as a basic medium, and further comprises 1.5 mg/L of the 6-BA and 0.6 mg/L of the NAA;
• and
• when the explant is derived from the Cathaya argyrophylla in the Huaping Town in Guilin, the proliferation medium uses the DCR medium as a basic medium, and further comprises 1 mg/L of the 6-BA and 0.4 mg/L of the NAA;
• inoculating the proliferated adventitious bud into a rooting medium to allow rooting culture to obtain a tissue culture seedling;
• wherein:
• the rooting medium uses a 1/2 MS medium as a basic medium, and further comprises 0.5 mg/L of indole-3-butyric acid (IBA) and 0.2 mg/L of the NAA;
• and
• the induction medium, the proliferation medium, and the rooting medium each further comprise the following components in mass percentage:
• 3% of sucrose and 4.5% % of agar.