Back to results
US 12,740,520 B2Granted
Mammal kinase inhibitors to promote in vitro embryogenesis induction of plants
AssigneeConsejo Superior De Investigaciones Cientificas (Csic)
InventorsPilar Sánchez Testillano, Ana Martinez Gil, Carmen Gil Ayuso-Gontan, Eduardo Berenguer Peinado, Elena Carneros García, Yolanda Pérez Pérez
Life of this patent
Estimating the term…
Abstract
The present invention relates to the use of mammal kinase inhibitors, preferably human kinase inhibitors, to promote the induction of in vitro embryogenesis, a strategy never used in plants systems before. The results obtained indicated that these inhibitors have beneficial effects in both crop and forest plants in in vitro systems of microspore and somatic embryogenesis.
Claims9
A method to induce in vitro plant embryogenesis, said method comprising:
- a. culturing microspores and/or explants in a culture medium suitable for embryo development;
- and
- b. adding mammal kinase inhibitors to the culture medium of a);
- and
- c. culturing for a period sufficient to obtain embryos,
- wherein the mammal kinases inhibitor is selected from a list consisting of:
- 4-benzyl-2-methyl-1,2,4-thiadozilidine-3,5-dione (TDZD8),
- 5-(2-Morpholinethylimino)-2,3-diphenyl-2,5-dihydro-1,2,4-thiadiazole (VP3.15),
- 3-acetyl-4-(1-methyl-1H-indol-3-yl)-1H-pirrol-2,5-dione (VP3.36),
- 4-hydroxy-1-ethyl-N′-palmitoyl-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (VP0.7),
- N-(6-methylbenzothiazole-2-yl)-4-morpholinobenzamide (JZ1.3),
- N-(6-fluorobenzothiazole-2-yl)-4-morpholinobenzamide, (JZ1.6),
- N-(6-bromobenzothiazole-2-yl)-4-morpholinobenzamide (JZ1.24) and
- (E,Z)-3-(morpholinoimino) indolin-2-one (IGS4.75).
11 more elements
Dependencies are inferred from the claim text.