Method for generating spectral data pertaining to microparticle sample, method for analyzing microparticles, method for distinguishing microparticles, method for assessing whether cancer-cell-derived exosomes are present, substrate for measuring spectrum of microparticles, device for measuring spectrum of microparticles, and apparatus for measuring spectrum of microparticles

An apparatus for acquiring spectral data of a microparticle sample containing a plurality of microparticles, the apparatus comprising:

• a device that includes a substrate having a through-hole;
• a guiding section for causing the microparticle to pass through the through-hole one by one;
• a light source;
• and
• a detecting section that acquires a measured spectrum by measuring light that is generated when light from the light source is applied to the microparticle in the through-hole, wherein
• the through-hole has a tapered structure in which a width continuously reduces from one surface of the substrate to another surface of the substrate,
• the through-hole has a shape of a quadrangular pyramid frustum,
• at least part of an inner surface of the through-hole is made of a metal that exhibits plasmon resonance,
• when the light source applies the light into the through-hole of the substrate, the guiding section causes the plurality of microparticles to pass through the through-hole one by one, and the detecting section acquires a measured spectrum from each of the plurality of microparticles, and
• the spectral data are a bundle of a plurality of the measured spectra.