Imaging-based system for monitoring quality of cells in culture

At least one non-transitory computer-readable storage medium having encoded thereon executable instructions that, when executed by at least one processor, cause the at least one processor to carry out a method of regulating treatment of a culture, the culture comprising a plurality of cells, cells in the plurality of cells having one or more respective cell categories selected from a plurality of cell categories, the plurality of cell categories including a first cell category and a second cell category, the method comprising:

• processing an image of the plurality of cells of the culture to identify, from among the plurality of cell categories, one or more cell categories of cells depicted in the image, the processing comprising:
• segmenting the image into a plurality of image segments by assigning individual pixels in the image to corresponding cell categories in the plurality of cell categories, the assigning comprising determining, for each of the individual pixels, a respective plurality of values corresponding to a respective plurality of cell categories, each of the respective plurality of values indicating a likelihood that the individual pixel corresponds to a cell of a respective cell category in the plurality of cell categories,
• wherein the plurality of image segments comprises:
• a first image segment comprising pixels associated with cells of the first cell category;
• and a second image segment comprising pixels associated with cells of the second cell category;
• determining, based on the plurality of image segments into which the image is segmented, an amount of cells in the culture corresponding to the first cell category;
• and
• regulating the treatment of the culture based on the determined amount of cells in the culture corresponding to the first cell category.

A cell imaging and incubation system, comprising:

• an imaging sensor configured to obtain an image of a plurality of cells of a culture;
• an incubator configured to incubate the culture;
• at least one processor;
• and
• at least one non-transitory computer-readable storage medium having encoded thereon executable instructions that, when executed by at least one processor, cause the at least one processor to carry out a method of regulating treatment of a culture, the culture comprising a plurality of cells, cells in the plurality of cells having one or more respective cell categories selected from a plurality of cell categories, the plurality of cell categories including a first cell category and a second cell category, the method comprising:
• processing an image of the plurality of cells of the culture to identify, from among the plurality of cell categories, one or more cell categories of cells depicted in the image, the processing comprising:
• segmenting the image into a plurality of image segments by assigning individual pixels in the image to corresponding cell categories in the plurality of cell categories, the assigning comprising determining, for each of the individual pixels, a respective plurality of values corresponding to a respective plurality of cell categories, each of the respective plurality of values indicating a likelihood that the individual pixel corresponds to a cell of a respective cell category in the plurality of cell categories,
• wherein the plurality of image segments comprises:
• a first image segment comprising pixels associated with cells of the first cell category;
• and a second image segment comprising pixels associated with cells of the second cell category;
• determining, based on the plurality of image segments into which the image is segmented, an amount of cells in the culture corresponding to the first cell category;
• and
• regulating the treatment of the culture based on the determined amount of cells in the culture corresponding to the first cell category.