Method for operating a microscopy system, microscopy system, and calibration method for a microscopy system

A microscopy system, comprising:

• a surgical microscope;
• at least one light source configured to irradiate a region to be examined;
• a modulator configured to modulate an amplitude of a luminous intensity of light emitted by the at least one light source;
• a beam guiding unit configured to guide, input couple, and collimate a beam from the at least one light source, the beam guiding unit including a plurality of optical elements;
• a control device configured to control the plurality of optical elements in the beam guiding unit;
• at least one of (i) a detector and (ii) a recording apparatus configured to detect the light emitted by the irradiated region to be examined, wherein the recording apparatus is arranged in the surgical microscope;
• a data memory;
• and
• a data processing unit,
• wherein the beam guiding unit is configured to steer the beam from the at least one light source in raster-like fashion over the region to be examined and to steer the light emitted by the irradiated region to be examined to the at least one of (i) the detector and (ii) the recording apparatus, and
• wherein, with the beam from the at least one light source, the data processing unit is configured to:
• (a) irradiate a region segment of a first region with the light emitted by the at least one light source at a first wavelength and the luminous intensity, wherein the amplitude of the luminous intensity is periodically modulated by the modulator at a modulation frequency,
• (b) determine a substance-specific parameter within the region segment as a response to being irradiated with the light from the at least one light source, and
• (c) repeat steps (a) and (b) for all region segments within the first region.