Microscope for determining properties of moving objects in a biological sample

A microscope for determining properties of moving objects in a biological sample, comprising:

• a sample space for arranging a biological sample;
• an objective for capturing a detection radiation coming from a confocal volume created in the sample;
• a detection beam path along which a captured detection radiation is steered to a detector in the form of a detector array having a plurality of detector elements, wherein the detector is arranged in a plane conjugate to a back-side image plane of the objective, and measurement values from the detector elements can each be read and analyzed on an individual basis;
• a light source for providing an excitation radiation and there being, in an illumination beam path, a beam-shaping optical unit for shaping the excitation radiation to form a confocal volume in the sample space and/or, in the detection beam path, a beam-shaping optical unit for shaping the detection radiation, and
• an analysis and control unit configured to use the detector to capture, at a plurality of times over a measurement duration, a detection radiation from a confocal volume created in the sample and to be able to in each case analyze the measurement values from the detector elements on an individual basis;
• and configured to select at least one pair of detector elements, wherein each partner of a pair is formed by at least one detector element;
• in a step B configured to create correlations of the acquired measurement values from the detector elements of at least one pair among themselves, wherein
• in a step B1 correlations are calculated starting from one of the selected detector elements in a first direction to the further selected detector element;
• and optionally
• in a step B2 correlations are additionally calculated starting from the further selected detector element in a second direction (back direction) to the first selected detector element;
• and
• in a step C configured to analyze the correlations in respect of the occurrence of a change in the intensity of the measurement values from selected detector elements.