Separating apparatus of biosubstance and separating method of the same

A separating method of biosubstance for processing a sample contained in a test tube, the sample including target biosubstances and non-target biosubstances, the separating method comprising:

• providing a controller to select a positive separation process or a negative separation process to execute according to types of the target biosubstances;
• providing a pipette pump to inject a first magnetic bead reagent or a second magnetic bead reagent into the sample according to the selected separation process, such that first immunomagnetic beads of the first magnetic bead reagent or second immunomagnetic beads of the second magnetic bead reagent binds to the target biosubstances;
• wherein, when the controller executes the positive separation process, the pipette pump is used to inject the first magnetic bead reagent into the sample, and when the controller executes the negative separation process, the pipette pump is used to inject the second magnetic bead reagent into the sample;
• providing a magnetic separation rack with two magnets having opposite magnetic properties, placing the test tube containing the sample on the magnetic separation rack such that the two magnets are arranged along a sidewall of the test tube with magnetic attraction surfaces facing toward the test tube, and applying a magnetic field to the sample by the two magnets to enrich the target biosubstances and separate the target biosubstances and the non-target biosubstances;
• and
• providing the pipette pump to separate the target biosubstances and the non-target biosubstances, wherein, when the controller executes the positive separation process, the pipette pump is used to suction and capture the target biosubstances, and when the controller executes the negative separation process, the pipette pump is used to suck away and remove the non-target biosubstances and leave the target biosubstances.