Back to results
US 12,736,541 B2Granted
Target-based method for high-throughput and subclass specific IgG glycan profiling in human plasma
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsYunlong Zhao, Shivkumar Raidas, Yuan Mao, Ning Li
Life of this patent
Estimating the term…
Abstract
This application relates to methods for identifying a glycosylated peptide biomarker in a sample. In particular, the application relates to methods for identifying an N-glycan profile of a protein in a sample.
Claims24
A method of identifying an N-glycan profile of a protein in a sample, comprising:
- enriching the sample using an affinity chromatography column to generate an enriched sample;
- contacting the enriched sample with one or more proteases to obtain a digested sample, wherein the digested sample comprises one or more peptides;
- loading the digested sample with a liquid chromatography column to provide an eluent;
- performing a multiple reaction monitoring on at least a portion of the eluent to obtain peptide backbone fragments using collision energy in a mass spectrometer;
- and
- analyzing the peptide backbone fragments to identify the N-glycan profile of the protein.
3 more elements
A method for identifying a glycosylated peptide biomarker in a sample, comprising:
- loading the sample on an affinity chromatography column;
- eluting an eluate from the affinity chromatography column;
- contacting the eluate with one or more proteases to obtain a digested sample, wherein the digested sample comprises one or more peptides;
- loading the digested sample with a liquid chromatography column to provide an eluent;
- performing a multiple reaction monitoring on at least a portion of the eluent to obtain peptide backbone fragments using collision energy in a mass spectrometer;
- and
- analyzing the peptide backbone fragments to identify the glycosylated peptide biomarker.
4 more elements
Dependencies are inferred from the claim text.