Method for detecting virus using SSDNA functionalized sensor
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsDebashis Chanda, Abraham Vazquez-Guardado
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Abstract
A method is for detecting a biomarker within a sample of blood. The method may include processing the sample of blood with a microfluidic blood plasma separator and a plasmonic array biosensor, and flowing the sample of blood over a sensing surface of the plasmonic array biosensor. The sensing surface of the plasmonic array biosensor may have an ssDNA aptamer against the biomarker. The method may further include binding the biomarker in the sample of blood to the ssDNA aptamer of the plasmonic array biosensor, and detecting the biomarker in the sample of blood based upon LSPR altering a reflected optical signal from the plasmonic array biosensor.
Claims20
A method for making a plasmonic array biosensor for detecting a biomarker within a sample of blood, the method comprising:
- positioning a microfluidic blood plasma separator on a substrate of a plasmonic array biosensor to process the sample of blood;
- and
- functionalizing the plasmonic array biosensor with a single stranded DNA (ssDNA) aptamer against the biomarker, a sensing surface of the plasmonic array biosensor to bind to the biomarker in the sample of blood, the plasmonic array biosensor to detect the biomarker in the sample of blood based upon a localized surface plasmon resonance (LSPR) signal, the functionalizing comprising
- cleaning the plasmonic array biosensor, and
- forming a hydrophilic film on the cleaned plasmonic array biosensor, the hydrophilic film comprising the ssDNA aptamer, polydimethylsiloxane, and polyvinyl alcohol;
- the biomarker comprising at least one of a DENV1-NS1 protein, a DENV2-NS1 protein, or a Dengue virus protein.
A method for making a plasmonic array biosensor for detecting a biomarker within a sample of blood, the method comprising:
- positioning a microfluidic blood plasma separator on a substrate of a plasmonic array biosensor to process the sample of blood, the microfluidic blood plasma separator being removably clamped onto the substrate;
- functionalizing the plasmonic array biosensor with a single stranded DNA (SSDNA) aptamer against the biomarker, a sensing surface of the plasmonic array biosensor to bind to the biomarker in the sample of blood, the plasmonic array biosensor to detect the biomarker in the sample of blood based upon a localized surface plasmon resonance (LSPR) signal, the functionalizing comprising
- cleaning the plasmonic array biosensor, and
- forming a hydrophilic film on the cleaned plasmonic array biosensor, the hydrophilic film comprising the ssDNA aptamer, polydimethylsiloxane, and polyvinyl alcohol;
- and
- passivating the sensing surface of the plasmonic array biosensor by at least forming a self-assembled monolayer;
- the biomarker comprising at least one of a DENV1-NS1 protein, a DENV2-NS1 protein, or a Dengue virus protein.
A method for making a plasmonic array biosensor for detecting a biomarker within a sample of blood, the method comprising:
- positioning a microfluidic blood plasma separator on a substrate of a plasmonic array biosensor to process the sample of blood, the plasmonic array biosensor comprising a hole-disc array comprising gold, the microfluidic blood plasma separator being removably clamped onto the substrate;
- functionalizing the plasmonic array biosensor with a single stranded DNA (ssDNA) aptamer against the biomarker, a sensing surface of the plasmonic array biosensor to bind to the biomarker in the sample of blood, the plasmonic array biosensor to detect the biomarker in the sample of blood based upon a localized surface plasmon resonance (LSPR) signal, the functionalizing comprising
- cleaning the plasmonic array biosensor, and
- forming a hydrophilic film on the cleaned plasmonic array biosensor, the hydrophilic film comprising the ssDNA aptamer, polydimethylsiloxane, and polyvinyl alcohol;
- and
- passivating the sensing surface of the plasmonic array biosensor by at least forming a self-assembled monolayer, the self-assembled monolayer comprising a thiol-terminated 6-mercaptohexanol self-assembled monolayer;
- the biomarker comprising at least one of a DENV1-NS1 protein, a DENV2-NS1 protein, or a Dengue virus protein.
Dependencies are inferred from the claim text.