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US 12,736,527 B2Granted
Functional cortical organoids, methods of making, and uses thereof
AssigneeThe Regents of the University of California
InventorsAlysson Renato Muotri, Priscilla Davidson Negraes, Cleber Trujillo, Pinar Mesci
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Abstract
The disclosure provides methods of making functional cortical organoids from somatic cells and stem cells and methods of using the functional organoids.
Claims14
An in vitro method of generating functional cortical organoids from neural progenitor cells (NPCs) comprising:
- culturing NPCs in serum-free supplemented neurobasal media comprising brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), neurotrophin 3 (NT-3), a fibroblast growth factor, L-ascorbic acid, and dibutyryl-cAMP to generate functional cortical organoids,
- wherein the functional cortical organoids are able to produce periodic and regular spontaneous network events upon differentiation, and a frequency and an amplitude of the network events increases over time.
Dependencies are inferred from the claim text.