Receptors for cyclic dinucleotides and methods for screening agonists or inhibitors thereof
AssigneeGuangzhou Winkey Technology Co., Ltd.
InventorsSujie Gu, Xiaobo Li
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Abstract
Disclosed herein is a recombinant vector being capable of expressing a novel receptor that senses exogenous cyclic dinucleotides (subtype M of STING) and meanwhile disclosed herein are a method and a kit for specifically identifying and detecting the expression of the said receptor. Further, provided herein are a medicament screening model and a kit for screening agonists or inhibitors targeting subtype M of the novel gene STING and the use thereof. Use of the screening model and the kit could simplify the screening procedure, enhance the screening efficiency and expand the range of the medicaments to be screened.
Claims6
A method for screening agonists or inhibitors for receptors of extracellular cyclic dinucleotides, comprising the following steps:
- culturing cells comprising a nucleotide sequence set forth in SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- adding medicaments to be screened to a culture medium in which the cells are cultured, incubating the cells, detecting the content of type 1 interferon or NF KappaB within the cells or cell culture medium, or monitoring a reporter fluorescein produced by the cells to which the medicaments are added by a photometer;
- and
- screening out the medicaments for increasing or inhibiting the expression of type 1 interferon or a reporter gene.
A cell line, comprising a subtype M gene of human STING or a subtype M gene of mouse STING, wherein:
- the subtype M gene of human STING is the nucleotide sequence set forth in SEQ ID NO:2, or is a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:4, and
- the subtype M gene of mouse STING is the nucleotide sequence set forth in SEQ ID NO:1, or is a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:3.
Dependencies are inferred from the claim text.