Electrochemical sensors based on enzyme-linked immunosorbent assay
AssigneeUnited States of America as represented by the Administrator of NASA
InventorsJessica Erin Koehne, Milton Jorge Santos Cordeiro, Morgan James Anderson
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Abstract
Electrochemical biological sensors, and more particularly electrochemical biological sensors based on enzyme-linked immunosorbent assay (ELISA) having a microbead detection construct coupled with magnetic immobilization construct. More particularly, Electrochemical ELISA-based biosensors comprising an electrode detection surface; a microbead detection construct; a magnetic immobilization construct; and an electrochemical reporter molecule.
Claims19
An electrochemical biosensor based on enzyme-linked immunosorbent assay, comprising:
- an electrode detection surface;
- a microbead detection construct comprising a plurality of signaling molecules;
- and
- a magnetic immobilization construct, wherein the magnetic immobilization construct comprises a magnetic microbead functionalized with a plurality of immobilization antibodies.
A method comprising:
- providing an electrochemical biosensor based on enzyme-linked immunosorbent assay, the sensor comprising:
- an electrode detection surface;
- a microbead detection construct comprising a plurality of signaling molecules;
- and
- a magnetic immobilization construct, wherein the magnetic immobilization construct comprises a magnetic microbead functionalized with a plurality of immobilization antibodies;
- exposing the sensor to a plurality of reporter molecules;
- contacting the sensor with a magnet;
- detecting the presence of one or more biological molecules based on one or both of an electrochemical signal or an optical signal from at least a portion of the plurality of reporter molecules.
A system comprising:
- an electrochemical biosensor based on enzyme-linked immunosorbent assay;
- a frame having a top and a bottom;
- a plunger comprising a solenoid and a magnet, wherein the magnet is located above the solenoid and toward the top of the frame, the plunger for vertically moving toward the top and toward the bottom of the frame;
- and
- a recess in top of the frame for receiving an electrochemical biosensor based on enzyme-linked immunosorbent assay,
- wherein vertically moving the plunger toward the top of the frame causes the magnet to interact with the electrochemical biosensor based on enzyme-linked immunosorbent assay when the electrochemical biosensor based on enzyme-linked immunosorbent assay is received by the recess, and wherein vertically moving the plunger toward the bottom of the frame prevents the magnet from interacting with the electrochemical biosensor based on enzyme-linked immunosorbent assay when the electrochemical biosensor based on enzyme-linked immunosorbent assay is received by the recess.
Dependencies are inferred from the claim text.