Multiplex nucleic acid detection system
AssigneeReditus Laboratories, LLC
InventorJoshua J. Geltz
Life of this patent
Estimating the term…
Abstract
A composition for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR) is provided. Also provided is a kit comprising the above composition with additional reagents for performing PCR using the plasmid, dye, primers and probes to detect the more than one target nucleic acid sequence. Additionally provided is a method for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR).
Claims2
A composition for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR), the composition comprising
- a plasmid comprising (a) a portion of each of the more than one target nucleic acid sequence, and (b) a sequence encoding a portion of a human housekeeping gene;
- a passive reference dye;
- and
- a set of two DNA primers and a DNA probe for each of the more than one target nucleic acid sequence and RNase P, wherein each of the probes comprise a dye,
- wherein the plasmid comprises SEQ ID NO:28 or 29.
A composition for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR), the composition comprising a plasmid comprising (a) a portion of each of the more than one target nucleic acid sequence, and (b) a sequence encoding a portion of a human housekeeping gene;
- a passive reference dye;
- and
- a set of two DNA primers and a DNA probe for each of the more than one target nucleic acid sequence and RNase P, wherein each of the probes comprise a dye,
- wherein the more than one target nucleic acid sequence is RNA from viruses selected from the group consisting of influenza A, influenza B, and SARS-COV-2;
- the plasmid comprises SEQ ID NO:28 or 29;
- the human housekeeping gene is RNase P;
- the passive reference dye is ROX;
- the set of two DNA primers for each of the more than one target nucleic acid sequence and the RNase P comprises SEQ ID NOs:
- 1-10.
Dependencies are inferred from the claim text.