Compositions and methods for detecting C1orf43 nucleic acid

A kit comprising:

• (a) a combination of oligomers comprising at least first and second amplification oligomers, wherein the first and second amplification oligomers are reverse and forward amplification oligomers, respectively, and are configured to specifically hybridize to first and second sites in the sequence of SEQ ID NO:
• 39 and generate an amplicon therefrom, respectively;
• wherein the first amplification oligomer comprises a target-hybridizing sequence that is from 14 to 50 bases in length and comprises the sequence of SEQ ID NO:
• 53 or SEQ ID NO:
• 54;
• wherein the second amplification oligomer comprises a target-hybridizing sequence that is from 15 to 40 bases in length and comprises the sequence of SEQ ID NO:
• 47 or SEQ ID NO:
• 48;
• and
• wherein at least one of the amplification oligomers is a promoter-primer and/or the combination further comprises at least one probe oligomer that comprises at least 10 nucleotides, is configured to specifically hybridize to an amplicon produced from the first and second amplification oligomers, and (i) comprises 2′-O-methyl-ribose in its backbone and/or (ii) comprises a non-nucleotide detectable label;
• and
• (b) an enzyme.