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US 12,735,742 B2Granted
Composition for nucleic acid analysis, nucleic acid analyzing method, and nucleic acid analyzer
AssigneeHitachi High-Tech Corporation
InventorsMichiru Fujioka, Yusuke Goto
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Abstract
In a base sequence determination system that performs a complementary strand synthesis reaction of a template DNA strand using DNA polymerase, a complementary strand synthesized by DNA polymerase is degraded by exonuclease in a solution.
Claims8
A nucleic acid analyzing method comprising the steps of:
- synthesizing a complementary strand of a nucleic acid to be analyzed by DNA polymerase in a solution including the nucleic acid to be analyzed, DNA polymerase, nucleotide as a substrate of DNA polymerase, and exonuclease;
- determining a sequence of one base in response to the synthesis of one base by the DNA polymerase;
- and
- degrading the complementary strand synthesized by DNA polymerase with exonuclease,
- wherein, in the sequence determination step, the DNA polymerase synthesizes the complementary strand of the nucleic acid to be analyzed in a first liquid tank among the first liquid tank and a second liquid tank that face each other with a membrane having a nanopore interposed therebetween such that the nucleic acid to be analyzed is moved in a direction from the second liquid tank to the first liquid tank through the nanopore to measure a signal generated during the movement of the nucleic acid to be analyzed, and
- wherein, in the degradation step, the nucleic acid to be analyzed for which the complementary strand is formed is moved in a direction from the first liquid tank to the second liquid tank such that the complementary strand is single-stranded and the single-stranded complementary strand is degraded by exonuclease in the first liquid tank.
3 more elements
Dependencies are inferred from the claim text.