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US 12,735,734 B2Granted
Low-cost, thermostable, lyophilized, cell-free protein synthesis platform
AssigneeNorthwestern University
InventorsMichael Christopher Jewett, Katherine F. Warfel
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Abstract
The present disclosure relates to stable, cost-effective, cell-free systems, compositions, methods, and kits for bio-manufacturing proteins. The systems, methods, and kits allow for cell-free bio-manufacturing of desired products in cell-free conditions, wherein the system can be stably stored or transported, in lyophilized form, at temperatures up to 50° C. for several weeks prior to use.
Claims12
A cell-free protein synthesis (CFPS) reaction composition comprising:
- (a) a cell lysate;
- (b) maltodextrin at a concentration of 10-500 mg/mL;
- (c) one or more buffer selected from HEPES, phosphate, and Bis-Tris;
- (d) added nucleotide monophosphates (NMPs).
1 more element
Dependencies are inferred from the claim text.